兔 anti-Pig IgG (Heavy & Light Chain) Antibody
ELISA, IHC, WB
Polyclonal
IgG
unconjugated
1 image
Quick Overview for 兔 anti-Pig IgG (Heavy & Light Chain) Antibody (ABIN101884)
抗原
IgG
克隆类型
多克隆
应用范围
ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
亚型
IgG
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抗原表位
- Heavy & Light Chain
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适用
- Pig
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宿主
- 兔
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Supplier Product No.
- 614-4102
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Supplier
- Rockland
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原理
- Swine IgG (H&L) Antibody
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交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Swine IgG, and Swine Serum.
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产品特性
- Anti-Swine IgG F(ab')2 Rhodamine Antibody generated in rabbit recognizes the dimeric Fab portion of the swine IgG molecule. Swine IgG F(ab')2 is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme pepsin under controlled conditions of temperature, time and pH . F(ab')2 Molecules lack the Fc portion of IgG and therefore receptors that bind swine IgG F(c) will not bind swine IgG F(ab')2 Molecules. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. This Anti-Swine IgG F(ab')2 antibody is conjugated to Rhodamine.
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纯化方法
- Anti-SWINE IgG (H&L) (RABBIT) Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Swine IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
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过滤
- Sterile filtered
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免疫原
- Optional[Immunogen]: Swine IgG whole molecule
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Goat anti-Pig IgG (Heavy & Light Chain) antibody (FITC)
WB, FACS, FLISA, FM, DB Polyclonal IgG FITC
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应用备注
- Application Note: Anti-SWINE IgG (H&L) (RABBIT) Antibody has been tested by ELISA and is suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. Specific conditions should be optimized by user. Immunohistochemistry Dilution: 1:1,000 - 1:5,000 Western Blot Dilution: 1:2,000 - 1:10,000 ELISA Dilution: 1:20,000 - 1:100,000 Other: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 8.8 mg/mL
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缓冲液
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Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
, Preservative:0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- IgG
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物质类
- Antibody
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背景
- SWINE IgG (H&L) (RABBIT) Antibody generated in rabbit detects specifically swine IgG whole molecule. Anti-Swine IgG antibody is ideal for investigators involved in serum protein component research.
抗原
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